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Fruit, Veg and Nuts

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FRUIT, VEG AND NUTS

Microbiology Laboratory NATA accreditation scope for Fruit, vegetables and nut foods

ServiceProductDeterminantTechniqueProcedure
Analysis for microorganismsBacterial isolates; Mould isolates; Yeast isolatesIdentificationMALDI-TOFIn-house method M39.0 using Bruker Library version BDAL-7311
 Fruit and fruit products; Gelatine and other gums; Herbs and spices; Honey; Mixed foods; Nuts and nut products; Vegetables and vegetable productsEscherichia coli​​​​; ColiformsSpread plate – Petrifilm              AOAC 051801 (in-house method M8.8.3)
  Campylobacter spp.; Listeria monocytogenes; Listeria spp.; Salmonella spp.Qualitative selective enrichmentBy the methods of – AS 5013.6, AS 5013.10, AS 5013.24.1
  Listeria spp.; Salmonella spp.Enzyme linked immunosorbent assay (ELISA) – SOLUSBy the methods of –   AFNOR SOL 37/01, AFNOR SOL 37/02
  Coliforms – Thermotolerant  Spread plate – PetrifilmAFNOR 3M-01-02-09-89-C (In-house method M8.7)
  Escherichia coli non O157 STEC serogroups O26, O45, O103, O111, O121, O145; Escherichia coli​​​​ O157; Salmonella spp.PCR – BAX SystemAOAC 2003.09, AOAC 091301, MLG 5B, MLG 5
  Escherichia coli​​​​; Coliforms – Thermotolerant and totalMost probable number (MPN)By the methods of – AS 5013.3, AS 5013.15
  Bacillus cereus; Listeria monocytogenes; Listeria spp.; Pseudomonas spp.; Staphylococcus spp. – Coagulase producing strainsSpread plate – Aerobic and anaerobicBy the methods of –   AS 5013.2, AS 5013.12.1, AS 5013.21, AS 5013.24.2
  Clostridium perfringens; Enterobacteriaceae; Lactobacillus spp.; Anaerobes; Coliforms; Lactic acid bacteria; Plate count; Yeasts and mouldsPour plateAS 5013.4, AS 5013.5, AS 5013.16, AS 5013.29, ISO15214, ISO 21528-2, In-house method M4.1, In-house method M10.2
  Enterobacteriaceae; Escherichia coli​​​​; Coliforms; Plate count; Yeasts and mouldsSpread plate – PetrifilmAFNOR 3M 01/06-09/97, AOAC 990.12, AOAC 991.14, AOAC 997.02, AOAC 998.08
  Listeria monocytogenes; Listeria spp.; Salmonella spp.Enzyme linked immunosorbent assay (ELISA) – Automated (VIDAS)By the methods of – AFNOR NF BIO-12/33-05/12, AOAC 071101,
  Escherichia coli non O157 STEC serogroups O26, O45, O103, O111, O121, O145; Escherichia coli​​​​ O157 H7         PCR – Assurance GDSBy the methods of – AOAC 2005.04, AOAC 071301
  Staphylococcus spp. – Coagulase producing strainsSpread plate – PetrifilmAFNOR 01/19 – 04/03 (M18.8)
 Fruit and fruit products; Herbs and spices; Nuts and nut products; Vegetables and vegetable productsListeria monocytogenesListeria spp.PCR – Multiplexin-house method M13.10 – Food Biotech, 29
  Salmonella spp.PCR – Multiplexin-house method M16.10 – Food Biotech, 29

Chemistry Laboratory NATA accreditation scope for Fruit, vegetables and nut foods

ServiceProductDeterminantTechniqueProcedure
Analysis for allergenic proteinsAlmond milk; Coconut milk; Ginger lollies; Orange and mango juice; Pecans; Potato chips; Soy (soya) milk; Swabs; Tomato juiceβ-LactoglobulinEnzyme linked immunosorbent assay (ELISA)              In-house CF120
 Almond milk; Coconut milk; Ginger lollies; Macadamia oil; Orange and mango juice; Pecans; Potato chips; Soy (soya) milk; Swabs; Tomato juiceCaseinEnzyme linked immunosorbent assay (ELISA)In-house CF121
 Almond milk; Coconut milk; Soy (soya) milk; Swabs; Tomato juiceMilk proteins – TotalEnzyme linked immunosorbent assay (ELISA)In-house CF111
 Orange and mango juice; Rice flour; Soy (soya) milk; SwabsLupin allergensEnzyme linked immunosorbent assay (ELISA)In house method CF092
 Potato chips; SwabsSesame allergensEnzyme linked immunosorbent assay (ELISA)In-house method CF119
 Ginger lollies; Macadamia oil; Pecans; Potato chips; SwabsGluten allergensEnzyme linked immunosorbent assay (ELISA)In-house CF040
 Potato chips; SwabsMacadamia allergensEnzyme linked immunosorbent assay (ELISA)              In-house method CF122
  Crustacean allergensEnzyme linked immunosorbent assay (ELISA)In-house CF117
Analysis for antioxidants, colourants, preservatives, other additivesFruit and fruit products; Jams; Juices; Nuts and nut products; Pickles; Vegetables and vegetable productsBenzoic acid; Sorbic acidHPLCIn-house CFH066
  Ascorbic acid (vitamin C)HPLCIn-house CFH061 and AOAC 967.21
 Fruit and fruit products; Jams; Vegetables and vegetable productsAscorbic acid (vitamin C)HPLCIn-house method CFH061 and AOAC 967.21
Analysis for elementsSoy (soya) milkIodineICP-MSIn-house method ESMI
 Fruit products; JamsCalcium; Copper; Iron; Magnesium; Manganese; Phosphorus; Potassium; Sodium; ZincICP-AESIn-house method ESI02 and MS016
  Aluminium; Antimony; Arsenic; Barium; Beryllium; Cadmium; Chromium; Cobalt; Copper; Lead; Lithium; Manganese; Mercury; Molybdenum; Nickel; Silver; Strontium; Thallium; Thorium; Tin; Titanium; Uranium; Vanadium; ZincICP-MSIn-house method ESM02
 Nuts and nut productsCalcium; Magnesium; Phosphorus; Potassium; SodiumICP-AESIn-house method ESI02
 Vegetables and vegetable productsCalcium; Copper; Iron; Magnesium; Manganese; Phosphorus; Potassium; Sodium; ZincICP-AESIn-house method ESI02
  Antimony; Arsenic; Barium; Beryllium; Cadmium; Chromium; Cobalt; Lead; Lithium; Mercury; Nickel; Selenium; Silver; Strontium; Thallium; Thorium; Tin; Titanium; Uranium   ICP-MSIn-house method ESM02
Analysis for herbicidesCitrus; Fruits(R)-2-(2,4-Dichlorophenoxy)propanoic acid (dichlorprop, 2,4-DP); Dichlobenil (2,3-dichlorobenzonitrile); OryzalinLC-MS-MSIn-house CR006.22; Other elements by in-house CR006.22, CR006CIT in accordance with MS016
 OnionsCyanazineGC-MS-MS; LC-HRMSIn-house CR006-ON
  Fluazifop (fluazifop-p-butyl); Ioxynil; Quizalofop; Quizalofop-ethyl; Quizalofop-p-terfurylGC-MS-MS; LC-HRMSIn-house CR006-ON
Analysis for mycotoxinsFruit juice concentrates; Fruit products;PatulinLC-MS-MS  In-house CR060.6 In accordance with MS016
 Nuts and nut productsAflatoxin B1; Aflatoxin B2; Aflatoxin G1; Aflatoxin G2LC-MS-MSIn-house CR060.1; Other mycotoxins by in-house CR060.a in accordance with MS016
  Aflatoxin B1; Aflatoxin B2; Aflatoxin G1; Aflatoxin G2HPLC; LC-MS-MSIn-house method CR060.2
Analysis for pyrethroid and pyrethrum pesticidesCitrus; FruitsCyfluthrin ([(R)-cyano-[4-fluoro-3-(phenoxy)phenyl]methyl] (1R,3R)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropane-1-carboxylate); Cyhalothrin (cyhalothrine); PhenothrinLC-MS-MSIn-house CR006CIT;
Other elements by in-house CR006.22, CR006CIT in accordance with MS016
Analysis for residues and contaminants – Hydrocarbons, phthalates, industrial chemicalsCoffee; Fruit juices; Fruits; Herbs; Honey; Legumes; Nuts; Vegetables10:2 Fluorotelomer sulfonic acid (10:2 FTSA, 1H,1H,2H,2H-perfluorododecane sulfonic acid); 4:2 Fluorotelomer sulfonic acid (4:2 FTSA, 1H,1H,2H,2H-perfluorohexane sulfonic acid); 6:2 Fluorotelomer sulfonic acid (6:2 FTSA); 8:2 Fluorotelomer sulfonic acid (8:2 FTSA, 1H,1H,2H,2H-perfluorodecane sulfonic acid); N-Ethyl perfluorooctane sulfanamido ethanol (EtFOSE); N-Ethyl perfluorooctane sulfonamide (EtFOSA); N-Ethyl perfluorooctane sulfonamido acetic acid (EtFOSAA); N-Methyl perfluorooctane sulfanamido ethanol (MeFOSE); N-Methyl perfluorooctane sulfonamide (MeFOSA); N-Methyl perfluorooctane sulfonamido acetic acid (MeFOSAA); Perfluorobutane sulfonic acid (PFBS); Perfluorobutanoic acid (PFBA); Perfluorodecane sulfonic acid (PFDS); Perfluorodecanoic acid (PFDA); Perfluorododecane sulfonic acid (PFDoDS); Perfluorododecanoic acid (PFDoDA); Perfluoroheptane sulfonic acid (PFHpS); Perfluoroheptanoic acid (PFHpA); Perfluorohexadecanoic acid (PFHxDA); Perfluorohexane sulfonic acid (PFHxS); Perfluorohexanoic acid (PFHxA); Perfluorononane sulfonic acid (PFNS); Perfluorononanoic acid (PFNA); Perfluorooctane sulfonamide (FOSA); Perfluorooctane sulfonate (PFOS); Perfluorooctanoic acid (PFOA); Perfluoropentane sulfonic acid (PFPeS); Perfluoropentanoic acid (PFPeA); Perfluorotetradecanoic acid (PFTeDA); Perfluorotridecanoic acid (PFTrDA); Perfluoroundecanoic acid (PFUnDA)LC-HRMSIn-house PFAS (CR148);
Other residues and contaminants in accordance with MS016
Analysis of fungicide residues and contaminantsAlmonds; Apples; Grains; Pears  FluquinconazoleGC-MS-MS; LC-MSIn-house CR071.1 and CR071.2
 Fruit productsDifenoconazole; Imazalil; ProchlorazLC-MS-MSIn-house CR006.2; Other elements by in-house CR006.22, CR006CIT in accordance with MS016
 Vegetables2-Chloro-N-([2-4-chlorophenyl]phenyl)pyridine-3-carboxamide (boscalid); Azoxystrobin; Benalaxyl; Carbendazim; Clorothalonil; Iprodione; Metalaxyl; Oxadixyl; Procymidone; TebuconazoleGC-MS-MS; LC-HRMSIn-house CR006-ON
  2-Chloro-N-([2-4-chlorophenyl]phenyl)pyridine-3-carboxamide (boscalid); Bupirimate; Captan; Carbendazim; Difenoconazole; Fenarimol; Fluquinconazole; Flusilazole; Imazalil; Iprodione; Kresoxim methyl; Myclobutanil; Penconazole; Thiabendazole; TrifloxystrobinGC-MS-MS; LC-HRMS              in-house CR006-AP
Analysis of pesticide residues and contaminantsCitrus; Fruit products(2-Dimethylamino-5,6-dimethylpyrimidin-4-yl) N,N-dimethylcarbamate (pirimicarb); 2,2,2-Trichloro-1,1-bis(4-chlorophenyl)ethanol (dicofol, kelthane); 2,2-Dichloropropanoic acid (2,2-DPA, dalapon); 2,4-Dichlorophenoxyacetic acid (2,4-D); 2-Chloro-N-([2-4-chlorophenyl]phenyl)pyridine-3-carboxamide (boscalid); 2-Phenylphenol (o-phenylphenol); Abamectin; Aldicarb; Aldrin; Azinphos-methyl (guthion); Azoxystrobin; Bifenazate; Bifenthrin; Bromacil; Buprofezin; Cadusafos; Carbaryl; Carfentrazone ethyl; Chlordane; Chlorpyrifos ethyl (chlorpyrifos); Clofentezine; Cypermethrin; Diazinon; Dichlorodiphenyltrichloroethane (DDT); Dieldrin; Diethyl-2-([dimethoxyphosphorothioyl]sulfanyl)butanedioate (maldison, malathion, carbofos, mercaptothion); Difenoconazole; Dimethoate; Diuron; Endosulfan; Endrin; Fenamiphos; Fenbutatin oxide; Fenthion; Fipronil; Fludioxonil; Heptachlor; Imazalil; Imidacloprid; Indoxacarb; Iprodione; Isoxaben; Metaldehyde; Methidathion; Methiocarb; Methomyl; Methoxyfenozide; Methylparathion (parathionmethyl); Norflurazon; Omethoate; Oxyfluorfen; Penconazole; Pendimethalin; Permethrin; Prochloraz; Profenofos; Pyrethrins; Pyrimethanil; Pyriproxifen (pyriproxyfen); Simazine; Spinetoram; Spinosad; Spirotetramat; Tebuconazole; Tebufenozide; Thiabendazole; Thiamethoxam; Trifloxystrobin; Trifluralin; γ-Hexachlorobenzene (γ-benzene hexachloride, γ-BHC, γ-HCB, lindane)GC-MS; LC-HRMS; LC-MSIn-house CR006CIT;
Other elements in accordance with in-house CR006.22, CR006CIT and MS016
 Herbs2-Chloroethanol (ethylene chlorohydrin)GC-MSIn-house CR058; Other residues by in-house CR058, CR059 in accordance with MS016
 OnionsChlorpyrifos ethyl (chlorpyrifos); Diazinon; Diethyl-2-([dimethoxyphosphorothioyl]sulfanyl)butanedioate (maldison, malathion, carbofos, mercaptothion); Dimethoate; Fenamiphos; Fenamiphos sulfone; Fenamiphos sulfoxide; Methidathion; Methylparathion (parathionmethyl); O,O-Diethyl S-([ethylsulfanyl]methyl) phosphorodithioate (phorate, thimet); Omethoate; Phorate oxone; Phorate sulfone; Phorate sulfoxide           GC-MS-MS; LC-HRMSIn-house CR006-ON
  Cinerin I; Cinerin II; Cyhalothrin (cyhalothrine); Cypermethrin; Jasmolin I; Jasmolin II; Pyrethrin I; Pyrethrin IIGC-MS-MS; LC-HRMSIn-house CR006-ON; Other elements by in-house CR006-ON in accordance with MS016
  2,2,2-Trichloro-1,1-bis(4-chlorophenyl)ethanol (dicofol, kelthane); Aldrin; Dichlorodiphenyldichloroethane (DDD); Dichlorodiphenyldichloroethylene (DDE); Dichlorodiphenyltrichloroethane (DDT); Dieldrin; Endosulfan I (α-endosulfan); Endosulfan II (β-endosulfan); Endosulfan sulfate; Endrin; Endrin ketone; Heptachlor; Heptachlor epoxide; α-Hexachlorobenzene (α-benzene hexachloride, α-BHC, α-HCB); β-Hexachlorobenzene (β-benzene hexachloride, β-BHC, β-HCB); γ-Hexachlorobenzene (γ-benzene hexachloride, γ-BHC, γ-HCB, lindane); δ-Hexachlorobenzene (δ-benzene hexachloride, δ-BHC, δ-HCB)GC-MS-MS; LC-HRMSIn-house CR006-ON; Other pesticides by in-house CR006-ON in accordance with MS016  
  Chlorthal dimethyl; Ethofumesate; Linuron; Metalochlor; Oxyfluorfen; Pendimethalin; PropachlorGC-MS-MS; LC-HRMSIn-house CR006-ON
  Methabenzthiazuron; Spinosyn A; Spinosyn DGC-MS-MS; LC-HRMSIn-house CR006-ON
  2-Aminobenzimidazole; DimethomorphGC-MS-MS; LC-HRMSIn-house CR006-ON
  3,4-Dichloroaniline; Methomyl; Methomyl-oximeGC-MS-MS; LC-HRMSIn-house CR006-ON
Analysis of physical and nutritional characteristicsFruit and fruit products; Fruit juice concentrates; Fruit juices; Jams; Nuts and nut products; Vegetable products;Dietary fibre – Insoluble; Dietary fibre – Soluble; Dietary fibre – TotalGravimetricin-house method CF057 and AOAC 985.29, 993.19
  Sulfur dioxideTitrationin-house method CF027 and AOAC 990.28
 Fruit and fruit products; Fruit juice concentrates; Fruit juices; Jams; Nuts and nut products; Vegetable products; VegetablesSugar profileHPLCIn-house CFH001.1        
 Fruit and fruit products; Jams; Nuts and nut products; Oils; Vegetable productsCholesterolGC-MSin-house method CFH064  
 Fruit and fruit products; Jams; Nuts and nut products; Vegetable productsWater activityVapour pressurein-house method CF032
 Fruit and fruit products; Jams; Nuts and nut products; Oils; Vegetable productsNitrogenKjeldahlin-house method CF002.2 and CF003.2
  ProteinKjeldahlin-house method CF002.1 and CF003.1
 Nuts and nut products; Vegetable productsMoistureGravimetricin-house method CF005.1 and CF005.2
 Fruit and fruit products; Jams; Vegetable productsAshGravimetricin-house method CF007  
 Fruit and fruit products; Jams; Nuts and nut products; Vegetable productsSucrose; SugarTitrationin-house method CF047
 Fruit and fruit products; Fruit juice concentrates; Fruit juices; Jams;Nitrate; Nitrite; Nitrite – Total as sodium nitriteFlow injection analyser (FIA)    In-house method CF020
 Fruit and fruit products; Fruit juice concentrates; Fruit juices; JamsTitratable acidityTitrationIn-house method CF012
 Fruit and fruit products; Jams;pHElectrometricIn-house method CF024
  Brix; Refractive indexRefractometryIn-house method CF022
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